rna fluorescence in situ hybridization kit  (Servicebio Inc)

Journal: Molecular Biomedicine

Article Title: YTH N6-methyladenosine RNA binding protein 2 mediated m 6 A modification of circHIPK2 promotes cellular senescence and osteoarthritis progression by inhibiting autophagy

doi: 10.1186/s43556-026-00441-4

Figure Lengend Snippet: Expression pattern of circHIPK2 and its characteristics in human chondrocytes. a , b Histological analysis, preoperative Kellgren-Lawrence and OARSI grading of sequencing cartilage sample (n = 3 per group). c Heatmap of circRNA expression in OA and NA chondrocytes. d RT-qPCR analysis of circHIPK2, circGOSR2, circREPS1, circMTUS1, circNUP54, circSLC8A1, circADAMTS6, circFN1, circTNFRSF21 and circAPBB2 expression in NA and OA chondrocytes ( n = 6 per group). e Representative images of circHIPK2 expression in NA and OA chondrocytes via FISH staining, γH2AX staining, SA-β gal staining. f CircHIPK2 expression pattern in chondrocytes treated with IL-1β, Etoposide and TNF-α, assessed via RT-qPCR ( n = 6 per group). g RNA fluorescence in situ hybridization (FISH) staining of circHIPK2 in aging mice and DMM mice. h Schematic illustration depicting HIPK2 exons 2 circularization to form circHIPK2. The presence of circHIPK2 was validated by RT-qPCR, followed by Sanger sequencing, red box represents head-to-tail circHIPK2 splicing sites. i RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with or without RNase R ( n = 6 per group). j RT-qPCR analysis of circHIPK2 and HIPK2 expression in chondrocytes treated with actinomycin D for different durations ( n = 3 per group). k RT-qPCR analysis of circHIPK2 and HIPK2 expression in NA and OA chondrocytes ( n = 10 per group). l Representative images of FISH staining for circHIPK2 localization. m RT-qPCR analysis of circHIPK2 expression in the nuclear and cytoplasmic fractions. GAPDH served as control. ** P < 0.01, *** P < 0.001

Article Snippet: A RNA fluorescent in situ hybridization kit (cat. no. GF-003, Servicebio, China) was used to detect circHIPK2 expression in chondrocytes and knee joint sections.

Techniques: Expressing, Sequencing, Quantitative RT-PCR, Staining, Fluorescence, In Situ Hybridization, Control

Journal: Frontiers in Pharmacology

Article Title: Gen-miR-5 derived from Gentianella acuta inhibits PFKP to prevent fibroblast activation and alleviate myocardial fibrosis

doi: 10.3389/fphar.2025.1578877

Figure Lengend Snippet: Gen-miR-5 specifically present in G.acuta might enter the mouse body after exogenous administration to mice (A) Flowchart of High-Throughput Sequencing and Bioinformatics Analysis. (B) Schematic illustration of in vivo imaging of Gen-miR-5 in mice. (C) miRNA was extracted from blood and cardiac tissues and treated with sodium periodate, followed by qRT-PCR to assess the expression levels of Gen-miR-5. Values are expressed as mean ± SEM. *** P < 0.001, **** P < 0.0001 compared to the corresponding control group (n = 4). (D) Gen-miR-5 in cardiac tissue was detected using RNA fluorescence in situ hybridization. Red fluorescence indicates Gen-miR-5 and blue fluorescence marks the nuclei. Scale bar = 50 μm. (E) H&E staining of mouse liver and kidney tissue sections. Scale bar = 2 mm.

Article Snippet: Gen-miR-5 and RNA Fluorescence in Situ Hybridization (FISH) assay kit were purchased from GenePharma (Suzhou, China).

Techniques: Next-Generation Sequencing, In Vivo Imaging, Quantitative RT-PCR, Expressing, Control, Fluorescence, In Situ Hybridization, Staining